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Alteromonas macleodii is a marine heterotrophic bacterium with widespread distribution − from temperate to tropical oceans, and from surface to deep waters. Strains of A. macleodii exhibit considerable genomic and metabolic variability, and can grow rapidly on diverse organic compounds. A. macleodii is a model organism for the study of population genomics, physiological adaptations and microbial interactions, with individual genomes encoding diverse phenotypic traits influenced by recombination and horizontal gene transfer. 

Abstract Telonemia are one of the oldest identified marine protists that for most part of their history have been recognized as a distinct incertae sedis lineage. Today, their evolutionary proximity to the SAR supergroup (Stramenopiles, Alveolates, and Rhizaria) is firmly established. However, their ecological distribution and importance as a natural predatory flagellate, especially in freshwater food webs, still remain unclear. To unravel the distribution and diversity of the phylum Telonemia in freshwater habitats, we examined over a thousand freshwater metagenomes from all over the world. In addition, to directly quantify absolute abundances, we analyzed 407 samples from 97 lakes and reservoirs using Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). We recovered Telonemia 18S rRNA gene sequences from hundreds of metagenomic samples from a wide variety of habitats, indicating a global distribution of this phylum. However, even after this extensive sampling, our phylogenetic analysis did not reveal any new major clades, suggesting current molecular surveys are near to capturing the full diversity within this group. We observed excellent concordance between CARD-FISH analyses and estimates of abundances from metagenomes. Both approaches suggest that Telonemia are largely absent from shallow lakes and prefer to inhabit the colder hypolimnion of lakes and reservoirs in the Northern Hemisphere, where they frequently bloom, reaching 10%–20% of the total heterotrophic flagellate population, making them important predatory flagellates in the freshwater food web. 

Summary This work aims at designing and implementing a system able to profile and help manage the set of Internet eXchange Points (IXPs) in an Internet region. As part of the Internet Society's strategy to help monitor and understand the evolution of IXPs in a particular region, a route‐collector data analyzer tool was developed before being deployed and tested in AfriNIC. In fact, traffic localization efforts in the African peering ecosystem would be more sustained, and their efficacy assessed if they were supported by a platform, which evaluates and reports in real time about their impact on the Internet. We, thus, built the “African” Route‐collectors Data Analyzer (ARDA), an open source web platform for analyzing publicly available routing information collected since 2005, by local route‐collectors. ARDA evaluates predefined metrics that picture the status of the interconnection at local, national, and regional levels. It shows that a small proportion of AfriNIC ASes (roughly 17%) are peering in the region. Through them, 58% of all African networks are visible at one IXP or more. These have been static from April to September 2017, and even February 2018, underlining the need for increased efforts to improve local interconnectivity. We show how ARDA can help detect the impact of policies on the growth of local IXPs or continually provide the community with up‐to‐date empirical data on the evolution of the IXP substrate. Given its features, this tool will be a helpful compass for stakeholders in the quest for better traffic localization and new interconnection opportunities in the targeted region.